Journal: International Journal of Molecular Sciences
Article Title: Renal Transcriptome and Metabolome in Mice with Principal Cell-Specific Ablation of the Tsc1 Gene: Derangements in Pathways Associated with Cell Metabolism, Growth and Acid Secretion
doi: 10.3390/ijms231810601
Figure Lengend Snippet: Analysis of 1 H NMR spectroscopic data. Intrinsic variations within and between the kidneys of Wt and Tsc1 KO mice were determined by ( A ) unsupervised PCA method demonstrating the magnitude of variation present in-between the groups and ( B ) supervised OPLS-DA analyses showing the separation between the kidney extracts of Wt and Tsc1 KO mice. Both ( A , B ) demonstrated a strong clustering within and significant separation between the test samples. ( C ) Metabolite heat maps of Wt and Tsc1 KO revealed significant changes in their metabolomes, the two bracketed areas represent the metabolomes that are significantly changed. Boxes have been drawn around the significantly altered metabolites. ( D ) List of significantly altered metabolites.
Article Snippet: Metabolites were assigned by comparing the chemical shifts with reference spectra found in databases, such as the Human Metabolome Database [ ], and Chenomx ® NMR Suite profiling software (Chenomx Inc., version 8.1).
Techniques: